Improved protocol for Agrobacterium mediated transformation of tomato and production of transgenic plants containing carotenoid biosynthetic gene CsZCD

Improved protocol for Agrobacterium mediated transformation of tomato (Lycopersicon esculentum) Micro-Tom was developed to use in corporation of the carotenoid biosynthetic genes CsZCD (Crocus zeaxanthin 7,8-cleavage dioxygenase). From these experiment, a transformation methodology using explants from cotyledons cultured for 1 day on the medium with zeatin 2 mg/L, IAA 0.1 mg/L, carefully submerged in the Agrobacterium inoculum for 20 min, then concultured with the agrobacterium for 3 days on the same medium, followed by a transfer to the same medium with 500 mg/L cefotaxin for 3 days and then by a transfer to the same medium with 100 mg/L kanamycin and 500 mg/L carabenillin for 6–8 weeks and resulted in a greater than 20% transformation efficiency in the concentration of Agrobacterium OD600 = 0.2 tested. In this transformation method, no feeder layers were used and the subculture media was minimal. Among the Agrobacterium concentrations of OD600 = 0.2, 0.5 and 1.0, the best transformation efficiency, 20.87%, was obtained by using OD600 = 0.2, which was significantly higher than that of OD600 = 1.0. The presence of the inserted target genes was checked using a rapid and efficient PCR test. The protocol was successfully employed in the production of transgenic Micro-Tom tomato containing the carotenoid biosynthesis CsZCD under constructive promoter. This procedure represents a simple, efficient and general means of transforming tomato.     

Profibus DP现场总线及其在火电厂的应用

现场总线作为过程控制领域的新技术，应用日益成熟和广泛。文章在论述Profibus DP总线通信协议和网络构成的基础上，分析了现场总线控制系统在火电厂应用的潜在问题和可行性，列举了Profibus DP应用实例。最后探讨了Profibus DP在国内首次应用的范围和深度。     

TCP/IP协议脆弱性分析

TCP/IP协议是网络发展的一个基础，但是该协议确存在着致命的弱点，盲目的使用与该协议相关的服务或应用会给网络安全带来隐患，认识到TCP/IP协议的脆弱性进对我们加强网络管理和采用安全技术手段具有指导性作用。     

OMNeT++仿真技术在计算机网络教学中的应用与研究

简要介绍网络仿真软件OMNeT ,分析OMNeT 在教学中应用的优点。通过OM-NeT 构建了一个模拟的尽量简化的计算机网络,并给这个网络配上原理性的网络协议,最后让它运行起来。这样可以让学生通过观看网络运作动画、分析网络性能结果和设计简单网络实体,更容易、深入地理解网络协议和算法的复杂行为,收到更好的教学效果。     

知识共享(CC)与数字图书馆的合作

版权问题一直制约着数字图书馆的发展,知识共享许可协议的使用可以帮助数字图书馆平衡版权保护和知识传播之间的关系.本文就知识共享(CC)与数字图书馆的合作进行了探讨.     

广西山区茶园无线网络监测系统设计

针对广西山区茶园设计了依靠节点自组织特性自动构建零通信费的ZigBee无线网络监测系统,能够定时采集茶园的空气温湿度、土壤温湿度和光照等参数。该系统除协调器外全部使用路由节点,种植区按蜂窝状划分,每个路由监测节点均布置在蜂窝中心,有效提高了监测系统可靠性。在种植区与监控中心之间的非种植区设计了路由中继节点,用于接力传送茶园监测数据至监控中心内的协调器。节点硬件电路包括核心板与底板,核心板设计了CC2530与RFX2401射频功放,底板设计了传感器处理模块、电源模块及调试接口。深入分析了Z-stack协议栈基于事件处理的多任务构架下用户程序设计方法。完成了山区茶园基于ZigBee技术的无线监控网络设计,实现了茶叶种植区内空气温、湿度及土壤温、湿度,光照参数,CC2530供电电压和工作温度的周期采集和传输。通过实际运行,整个系统稳定可靠。     

一种水下无线传感网络中节能跨层路由协议

为了提高水下无线传感器网络（Underwater Wireless Sensor Network,UWSN）的能量有效性,对节能路由策略进行研究.当给定源宿节点距离,通过多跳中继转发数据时,证明了一定存在能量最优的中继跳距.提出了一种节能的路由协议,节点通过适当的信息交换来获取邻居信息而不必知道全局信息,基于跨层设计的思想,在物理层根据反馈信息和最优跳距分配不同的功率级,在网络层寻找与理想路径临近的节点作为下一跳节点.仿真结果表明,该协议相对于FBR、VBF和DFR路由协议消耗更少的能量.     

计算机与变频控制器网总线通讯技术应用

以无锡市中桥水厂沉淀池计算机与变频器控制为例，探讨了计算机与多台变频器之间的总线通讯，实现对变频控制器内部参数的设定和对其进行监控；研究了总线通讯软硬件实现方法和提高通讯可靠性及效率的措施。提高了变频器的控制能力和控制范围，节省工程成本。     

Carbon sequestration in the agricultural soils of Europe

In this review, technical and economically viable potentials for carbon sequestration in the agricultural soils of Europe by 2008-2012 are analysed against a business-as-usual scenario. We provide a quantitative estimation of the carbon absorption potential per hectare and the surface of agricultural land that is available and suitable for the implementation of those measures, their environmental effects as well as the effects on farm income. Realistically, agricultural soils in EU-15 can sequester up to 16-19 Mt C year⁻¹ during the first Kyoto commitment period (2008-2012), which is less than one fifth of the theoretical potential and equivalent to 2% of European anthropogenic emissions. We identified as most promising measures: the promotion of organic inputs on arable land instead of grassland, the introduction of perennials (grasses, trees) on arable set-aside land for conservation or biofuel purposes, to promote organic farming, to raise the water table in farmed peatland, and—with restrictions—zero tillage or conservation tillage. Many options have environmental benefits but some risk of increasing N₂O emissions. For most measures it is impossible to determine the overall impact on farm profitability. Efficient carbon sequestration in agricultural soils demands a permanent management change and implementation concepts adjusted to local soil, climate and management features in order to allow selection of areas with high carbon sequestering potential. Some of the present agricultural policy schemes have probably helped to maintain carbon stocks in agricultural soils.     

农村公务员的考核方法研究

采用系统工程理论中的层次分析法,应用考核软件通过计算,得到在考核层总排序中所有被考核公务员的量化数据,从而为农村公务员考核提供科学、公正、客观的考核参考数据。